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As sorbitol solutions had only restricted influence on cells, we excluded them from further more experiments. For move cytometry, Caco-two cells ended up stained with propidium iodide and labelled with annexin V to tell apart between necrotic and early apoptotic cells.

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= 0.0403) experienced important correlation with overall larvae mortality. Macromolecules are not often examined On this new model organism, yet in high focus they demonstrate concentration dependent toxicity [forty seven]. Labelled annexin V was utilised as an indicator of early phase apoptosis, and we have discovered that the proportion of stained cells had no correlation with osmolality.

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